FastQCFastQC Report
Thu 28 Apr 2016
ERR1042416.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042416.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5078391
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT402340.7922588079570871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT377720.743778885871529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT280010.5513754257992345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168100.3310103534761305No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC111480.21951834744508644No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG86250.1698372575093174No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA68740.13535783282539685No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA53270.10489542849300103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA4300.016.34883716
ACGAACG4750.015.18947315
TCGTTTA7700.015.13636330
GACGGAC11350.014.8325997
CTAGCGG6150.014.73983929
ACGGACC11200.014.7008928
AAGACGG12350.014.3805675
TCTAGCG6200.014.3225828
TAACGCC8750.014.1657144
GTAAACG6050.014.06611627
TTAACGG3450.013.94202835
TATACCG3200.013.8750015
TACGACG7750.013.8451625
TCGAACG3400.013.60294153
CGAGCCG10400.013.51923115
CGCATCG8650.013.47398813
TAACGAA5250.013.39047513
GTATTAG14700.013.3401361
CGTTAAT1254.850757E-513.3200011
GTATAGA5600.013.2142851
Produced by FastQC (version 0.11.5)