FastQCFastQC Report
Thu 28 Apr 2016
ERR1042414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7388996
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1008221.3644884907232322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT970841.3138997503855734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT731720.990283388974632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417030.5643933221780063No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA129970.1758966982794415No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT121720.16473144659978162No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA115400.15617818713124218No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110970.15018278532022483No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA94970.1285289638808845No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85460.11565847376287658No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84730.11467051815970669No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA79760.10794429987511157No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA75250.10184062895689752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAA1802.0190782E-1015.41666614
AAGACGG16500.015.1363645
TCGTTTA13900.015.03956830
ACGGACC15700.014.6114658
GACGGAC16500.014.3515167
TTAACGG5350.014.17756935
TCTATAC6950.014.1079143
CGAACGA10100.014.10396116
GTATTAG20400.014.0563731
TCTAGCG10700.014.00467328
CTAGCGG10750.013.93953429
TAACGGC5350.013.83177636
ATCGTTT16100.013.7888229
CGGTCCA11900.013.68067210
CGCAAGA17750.013.6535212
CGAGCCG17200.013.44476715
TAACGCC17550.013.3874654
ATGGTCG14100.013.38297836
CGGACCA18950.013.374679
TCTTATA36500.013.22876637
Produced by FastQC (version 0.11.5)