FastQCFastQC Report
Thu 28 Apr 2016
ERR1042413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5010979
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1506183.005759952296747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1275682.545769998237869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT911261.818526878679795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528401.0544845627970103No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA201380.40187755725976904No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT150830.3009990662503276No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA148740.29682822458445746No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA130640.2607075383872094No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA129490.25841257766196984No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA126300.2520465561719576No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125100.24965181454562071No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA106090.21171511594840048No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102340.20423154836609775No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98840.19724688528928178No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC97910.1953909605288707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG83370.1663746744897554No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA78590.15683562034484677No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78000.1556582057118978No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.12384805444205613No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA61800.1233291937563498No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA61540.12281033307064348No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA60900.12153313753659714No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA59830.1193978262531134No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA59350.11843992960257867No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57180.11410943849495278No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA56720.11319145420485698No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA54810.10937982378293742No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52960.10568793044233471No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA51940.10365240005994836No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAT701.220235E-418.56
TCTAACG2300.017.6956522
ACGTTTA3050.017.59016426
TTAACGG3500.017.44285635
CTAACGC2500.017.023
TCTTATA30000.016.77333337
CAATGCG3400.015.77941119
CGGTCCA2750.015.47272810
CTAGCGG2750.015.47272829
CGACGAG3600.015.41666624
TCGCTAA2300.015.28260914
TGCGACG3400.015.23529322
ATTAGAG7300.015.205483
TATACTG5500.014.85
GTCGTCA3500.014.79999924
GTATTAG11850.014.5189871
TCTAGCG2950.014.42372928
CTGTGCG5150.014.3689329
TAACGGC4250.014.36470636
TAATACT9400.014.1702144
Produced by FastQC (version 0.11.5)