FastQCFastQC Report
Thu 28 Apr 2016
ERR1042412.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042412.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6320727
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT815331.289930731069385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT813391.2868614638790759No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT629270.9955658581678974No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338710.535871902077087No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT97480.15422276583057615No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87960.13916120724720432No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA81350.12870354944929593No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76490.12101456050862504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66770.10563658262728322No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63340.10020999166709778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5500.017.15454735
TAATACT16000.015.3781244
ATTAGAG13050.015.1685823
TAACGGC6000.015.10833436
GACGGAC9100.015.0439567
AACCGTA2251.8189894E-1214.87
CGGACCA9450.014.682549
ACGGACC9500.014.6052638
TACCGTC7050.014.4326247
CGTCGTA6300.014.38888910
CGAACGT2750.014.1272744
GGTATCA415950.013.8811151
CTAGCGG6200.013.72580729
TATTAGA13400.013.6679112
GCGCTAT2051.4279067E-913.53658519
CGCAAGA10800.013.5324082
GTATTAG19050.013.4986881
AAGACGG10300.013.4708755
ATACGAC1102.4591023E-413.4545453
ACACGCT16550.013.1903329
Produced by FastQC (version 0.11.5)