FastQCFastQC Report
Thu 28 Apr 2016
ERR1042411.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042411.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4742835
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT969682.044515569274495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT956892.0175485759044958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT727681.5342722232588737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444690.937603774957383No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC143450.3024562313468632No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT132480.2793266052898741No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA123980.26140483487197No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123850.261130737206755No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG105230.22187151777365224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104230.2197630741950753No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA99090.2089256742011898No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91610.19315451623343421No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA88790.18720870534184722No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA85070.17936529522954098No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84570.1783110734402525No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA82120.173145386672739No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA65550.13820847657571894No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG64780.13658497502021472No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT59190.12479877541596958No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC57770.1218047855343903No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG56710.11956983534109873No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55040.11604873456487522No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53870.11358185557794019No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA53230.11223245168765095No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG48670.10261794896934007No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT47620.10040408321183426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4100.017.5975635
TTAACGG4700.017.31914935
TAACGGC5100.015.96078436
CGTTCGG700.002594097315.85714224
ATTAGAG7550.015.1920543
GACGGAC5750.015.1217397
TATTAGA9200.015.0815222
CAAATCG4550.015.04395613
TCTATAC4500.014.83
GTATTAG13150.014.20912551
TTAGCGA3650.014.19178227
CGTGCGC6400.014.16406310
ACGTGCG5900.014.1101699
TAGCGAG3700.014.028
TAATACT12250.013.5918374
ACGGACC6400.013.5859388
CTCTAAT10100.013.5544561
CGAGCCG7250.013.52413815
AACACGT5850.013.2820526
GTACTAG1951.0284566E-813.282051
Produced by FastQC (version 0.11.5)