FastQCFastQC Report
Thu 28 Apr 2016
ERR1042410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3707728
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1193553.219087268537498No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1151033.1044078745797963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT845692.2808846819399915No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT533111.4378347063214993No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC227780.6143384843764159No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA186180.5021403943331334No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG172090.46413868546991577No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT159070.4290228409419461No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145590.392666344456767No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA141550.3817701837891021No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA136100.3670711551656432No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA121470.32761302878744075No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118430.31941393759197007No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA113770.30684559385154464No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104020.28054916649765027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103450.27901183689849957No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG102150.275505646584647No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG96250.25959293669870065No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA92320.2489934536729771No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC87530.23607449090116642No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80100.21603526472276283No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG75070.20246900527762557No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA73450.19809975273267078No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA73270.19761428022767583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT71190.19200437572551168No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.17951694406925212No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA65300.1761186365342873No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65300.1761186365342873No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA65110.1756061933345704No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA61350.16546521211911985No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA59320.15999016109056544No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT54460.14688240345570117No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA53680.14477868926738963No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50740.1368493050191384No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA47920.1292435691075505No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA46600.12568343740425403No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC45660.1231481921003914No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA45450.1225818075112306No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA45220.12196148153262591No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG44960.1212602434698554No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA44530.12010050359681185No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC42070.1134657126952139No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC42010.11330388852688222No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA41440.11176655892773148No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA40950.11044499488635628No Hit
GAGTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAA39530.10661515623584038No Hit
GTATCAACGCAGAGGACTTTTTTTTTTTTTTTTTTTTTTTTTT39430.10634544928862097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG38770.10456538343697273No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA38670.1042956764897533No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA38420.10362140912170473No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA37730.10176043118589066No Hit
ACGCAGAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAA37500.10114010520728597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG1201.0430085E-716.95833422
CACGCAG3050.016.377055
TCTTATA30200.016.11092837
CCGCCTA700.002593952715.85714315
GCAGTCG3300.015.6969712
AATCGTC1302.593315E-715.65384628
ACACCGT957.066454E-515.5789486
TCTAACG855.3681515E-415.2352942
CAATGCG1353.9787847E-715.07407319
AATACTG4300.015.0581415
GAGGACT21750.015.0551741
ACGGACC1001.0944065E-414.88
CAGTCGT3400.014.69117613
GACTTTT22250.014.6337094
GTACTAG1651.6485501E-814.5757571
ACCCGTT1651.6485501E-814.57575730
GGGTTAG4200.014.5357147
TAATACT5750.014.4782614
ATCCGTA1956.7484507E-1014.2307712
CTAACGC1051.6572002E-414.0952383
Produced by FastQC (version 0.11.5)