FastQCFastQC Report
Thu 28 Apr 2016
ERR1042409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5905636
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1359042.301259339383599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1311772.2212171559506886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT944271.598930242229626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT606911.0276793219223128No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA190990.32340293238526724No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT182610.3092130974547026No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169080.28630277924342107No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA159460.2700132551345867No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA148990.25228442796000294No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128070.21686063956532373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC127980.2167082427701267No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127310.21557373329477128No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA124670.2111034273023261No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA122670.20771683185350404No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109780.18589022418584553No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG99710.16883871610102621No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA81890.1386641506520212No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA81240.13756350713115403No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA80580.13644593063304272No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.13101044493768324No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT76620.12974047164437497No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75110.12718359208051427No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA72100.122086765930037No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA71810.12159570958995779No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA70040.11859857261775023No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA66000.11175764981112958No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG66000.11175764981112958No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA62910.10652535984269941No Hit
GTACATGGGAAGCAGTTGTATCAACGCAAAAAAAAAAAAAAAA60010.10161479644190735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG5750.016.08695615
TTAACGG3700.015.50000135
TATACTG7100.015.1126775
CGAACGA4100.014.890244516
TAACGAA4350.014.885056513
TACGACG4500.014.85
TGCGACG1001.09458124E-414.822
TACTCGC2650.014.660377520
TCTTATA39650.014.60403537
TAACGGC4100.013.98780536
GACGGAC6100.013.950827
GTATTAG11200.013.8750011
ACGGACC6300.013.8015878
CGTATTA2950.013.7966115
ATGCGTG2900.013.3965517
TCTATAC5250.013.3904753
CTTATAC24700.013.10728837
CGGACCA6900.012.8695659
GACGGTA5350.012.7943938
TCCGTAT3500.012.68571513
Produced by FastQC (version 0.11.5)