FastQCFastQC Report
Thu 28 Apr 2016
ERR1042407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8264166
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT888291.0748695028633257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT740580.8961339837558926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT611050.7393970547058227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370220.4479822888359212No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC195430.2364787928993682No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG146890.17774328347228263No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA122670.14843603093161487No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA121180.1466330661799388No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT102650.12421096091245021No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99100.1199153066383226No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91950.11126349591719237No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA83930.1015589473880365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA803.385736E-416.187519
CAAGACG22000.015.8931814
AAGACGG21750.015.6505755
CGAACGT4500.015.6222224
GACGGAC20950.015.4534627
ACGGACC21100.015.431288
CTAGACT6050.015.2892564
TCGAACG4800.014.6458343
CGGACCA22650.014.620319
CGCAAGA22800.014.3618432
CGAGCCG17650.014.15014215
GCGCAAG23950.014.1356991
TCTAGCG11200.013.87528
TCGCGAA1356.5782842E-613.70370420
TATACTG13850.013.6245495
CGATGCG14900.013.53355733
AGACGGA24450.013.4683026
TACCGTC17350.013.4351587
CGAACGA18150.013.25068916
CTAGCGG11850.013.11392429
Produced by FastQC (version 0.11.5)