FastQCFastQC Report
Thu 28 Apr 2016
ERR1042406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4302265
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT640481.488704205807871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT541651.2589879981823526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT386000.8972018227608016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253390.5889688338584443No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC152180.3537206564449191No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG117160.27232167242138733No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA98930.22994864333089662No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA89110.20712345706273322No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77620.18041659451474978No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG68560.1593579196074626No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG62020.1441566244757122No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60920.1415998317165493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC55820.12974561074224855No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52600.1222611810290626No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG52600.1222611810290626No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA51350.11935573471183203No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51270.11916978614752927No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50970.11847247903139393No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47870.11126697216466211No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA43120.1002262761591859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACG7450.015.14765127
CGAACGT3550.015.1126774
CGCAAGA13250.014.8000012
AAGACGG12750.014.85
CGACGTT1001.0944718E-414.79999924
TCGAACG3650.014.6986313
ACGCTTC7900.014.51898731
TCTAGCG5250.014.447618528
TACGGTT908.2805194E-414.3888895
TTCGGGC7850.014.37579635
ACTCTAA8900.014.13483210
ATTAGAG8250.014.1272743
ACCGCGT1051.6572974E-414.0952398
TTAACGG2101.364242E-1014.09523935
TTACGTA1051.6572974E-414.0952394
ATAACGA9200.014.07608712
CTTCGGG8550.014.06432734
CACTCTA8950.014.0558669
GCGCAAG13750.013.9927271
TAGTGCG2252.7284841E-1113.97777759
Produced by FastQC (version 0.11.5)