FastQCFastQC Report
Thu 28 Apr 2016
ERR1042405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760402
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC57010.7497350085875629No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG52920.6959476697851925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39940.5252484869845161No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA37870.4980260441187687No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG33430.4396358768125281No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25040.32929950210546527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.32114592018432353No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG20330.26735858138195323No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18320.2409251948311551No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC17780.2338236879966123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC16230.21343973319375803No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT15190.19976275706797192No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG13970.18371861199733824No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12590.1655703167535067No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA11610.1526823969426698No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11130.1463699464230762No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT9570.12585448223439705No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA8510.11191448733696124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.10954731839211364No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8200.10783769637639037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAC656.8982044E-519.9230773
GTGCTAC609.232898E-418.51
GTATATG701.21850855E-418.51
TACGTCG650.001579269217.0769235
TAAGAGT1107.7990626E-716.8181824
TAAACTC803.3806826E-416.18755
GTACTAT803.3806826E-416.18756
GTCGAGA700.002591411415.8571431
AGGACCG1403.4671757E-815.8571435
TTACACC1403.4671757E-815.8571434
GGTTCTA1059.334703E-615.85714213
ACGGACC1302.5854024E-715.6538468
CCTTAGT957.054654E-515.5789481
CTAGCGG957.054654E-515.57894829
CGCAATA1201.9333074E-615.41666636
TAATACC855.3611863E-415.2352944
TAATACT1101.4505049E-515.1363644
GTATTGG1101.4505049E-515.1363641
CGCGGTT1101.4505049E-515.13636410
AAGACGG1853.0377123E-1015.05
Produced by FastQC (version 0.11.5)