FastQCFastQC Report
Thu 28 Apr 2016
ERR1042404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102373
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT250891.1933657823801962No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC172230.819217141772654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168360.8008093711249146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143260.6814204710581804No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG112370.5344912629680841No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA107190.5098524381734354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67620.3216365506977116No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC67340.3203047223304333No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG63830.30360930244062306No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG55320.26313123313512876No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT46130.21941872350910138No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA37460.17817960942230515No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC36220.1722815123672155No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG35690.169760551529153No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT28480.13546597107173655No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28320.13470492629043465No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC27370.13018622290145468No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.12309899337558083No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.11938890006673412No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG24010.11420428249411499No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG22330.10621331229044512No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC22200.10559496340563734No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA21820.10378748205004534No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG604.3264663E-827.74999835
CGTATTA1107.4578566E-1121.86363615
CTAATCG1151.3460522E-1020.91304426
CGTCTAA500.00703677518.51
TCTAGCG902.1539436E-618.528
AATCGTC1401.87174E-917.17857228
TAACGGC904.4487784E-516.44444536
CGGACGT904.4487784E-516.44444536
TACGACG2200.015.9772735
GTATAGG4900.015.4795911
CGACGGT2400.015.4166667
TCTATCG1455.3518306E-815.31034531
TATCGTG1101.452928E-515.13636437
TACTCGC1353.9764018E-715.07407520
GACGGAC2750.014.87
CGCATCA1651.6470949E-814.57575724
ACGACGG2550.014.5098046
GTACGTA1304.447893E-614.23076913
TCGTCCC1702.4396286E-814.14705930
CGTCCCA1702.4396286E-814.14705931
Produced by FastQC (version 0.11.5)