FastQCFastQC Report
Thu 28 Apr 2016
ERR1042403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3153933
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT183460.5816864213665921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106820.3386882346581237No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC99290.3148132823366888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95050.30136975008663786No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA75160.23830563299854496No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG66820.2118624587142466No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG45680.1448350361279076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.1404912533018298No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG41810.1325646423053375No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT35670.11309688569795236No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC32260.10228498829873685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT2850.018.1754384
AACCGTA650.001580760317.0769237
CGAACGA4250.015.23529416
TCCGATA4500.015.2111118
AAGACGG8250.015.0242435
GTATACG1406.00392E-714.5357151
CCGATAA4750.014.4105279
TTAACGG1051.6570758E-414.09523935
TCTACAC5150.014.0097083
TAATCGG1601.7863385E-713.87516
CGCATCG5700.013.30701713
ATAACGA5150.013.29126212
ATTAGAC2651.8189894E-1213.264153
TCGCTAA850.00940948413.05882414
TACACCG2850.012.9824565
ACGAACG5000.012.9515
GACGGAC8350.012.8502997
CGAACGC1307.005357E-512.80769330
TTAGAGT6500.012.8076934
CGGACAT6950.012.776978521
Produced by FastQC (version 0.11.5)