FastQCFastQC Report
Thu 28 Apr 2016
ERR1042402.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042402.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479797
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT88090.5952843531916878No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC59190.39998729555472806No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA53640.36248215126804556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.34815586191889836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32860.22205748491178182No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30420.205568736792952No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG26220.1771864654408679No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24790.16752297781384878No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.15698099131164614No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23120.15623764610956772No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21950.14833115623291573No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG16480.11136662663865382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2300.020.91304416
TCCGATA2300.020.1086968
CCGATAA2350.019.680859
ACGAACG2500.019.2415
AACGAAC2700.017.81481614
CGATAAC2700.017.81481610
AATTCCG2600.017.7884625
TATACTG2450.017.3673465
TTCCGAT2800.017.1785727
ATAACGA2700.017.1296312
TAATCGT650.001580224317.0769234
GCTATAC1005.8800906E-616.653
GATAACG2950.016.30508411
TAACGAA2850.016.2280713
GTAATAC1659.749783E-1015.6969713
ATTCCGA3300.015.6969716
CCGTCGT4500.015.6222229
TACCGTC4500.015.6222227
CGTCGTA4550.015.45054910
ACCGTCG4450.015.3820238
Produced by FastQC (version 0.11.5)