FastQCFastQC Report
Thu 28 Apr 2016
ERR1042401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5178309
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC366680.7081076081014092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316630.611454434256434No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA232820.44960623245928355No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG227100.4385601554484292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT213140.4116015479184421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT185630.3584760971197354No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC147400.28464890758739964No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG129460.2500043933260839No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG110480.21335150142643092No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97430.1881502243299888No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT96240.1858521768399684No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA77650.14995242655469188No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG74120.14313552937841292No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC73090.14114646306352133No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT58830.11360851583016773No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57150.11036421349131542No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG56870.10982349643484002No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC54910.10603847703951233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG8200.014.4390245
AAGACGG11300.014.0796465
CGTTATA1854.9112714E-914.02
ACGGACC11150.013.77138
GACGGAC11100.013.6666677
CGACGGT8100.013.2469147
TACGACG8250.013.0060615
ATATCGT1000.001833989212.9499996
TAATACT10550.012.8009474
GTATTAG13450.012.79182151
CAAGACG14050.012.7722434
GTATAGG9450.012.7248681
CGGACCA12650.012.723329
TCTACAC8900.012.6797763
ACCGTCG8700.012.3333348
TTAGACT4800.012.3333334
ACGACGG8750.012.2628576
TACCGTC8600.012.2616277
CGCAAGA12250.012.2326532
CTACACT14650.012.1228674
Produced by FastQC (version 0.11.5)