FastQCFastQC Report
Thu 28 Apr 2016
ERR1042400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219346
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC573191.7804547880221635No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG413721.2851057326550175No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA364781.1330872792175803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT331941.031078983122659No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG241670.750680417699744No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC224950.698744403366398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT206270.6407201959652674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202330.6284816854106393No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG170340.5291136771257268No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT151150.46950529703859106No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC126450.39278163950069356No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG113990.35407812642692027No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA107810.3348816809376811No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT101800.3162132930104437No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT91310.28362903521398447No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89430.277789339822436No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC87710.2724466397833597No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG78300.24321710061608787No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG72680.22576013886050147No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT69330.21535429866811456No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC62750.19491536479769495No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA61710.19168489500662558No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG61530.19112577523509433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC60410.18764680776778886No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC55750.17317181812703575No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50250.15608760288580353No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC49220.15288819530426365No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC47960.148974356903545No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT47860.14866373480824988No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT46070.14310359930246702No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT44700.13884807659692372No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT43200.13418874516749676No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA42940.1333811277197294No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT42710.1326666969005506No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT42520.13207651491948985No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG41900.13015065792866004No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA40390.12546026428970355No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT39060.12132899042227832No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC38630.11999331541250925No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG38460.11946525785050753No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37840.1175394008596777No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT37360.11604841480226107No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.11188607872530633No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT35080.10896623102953208No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34330.1066365653148186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACG2400.022.35416822
CAATGCG2950.019.44067819
CGACGAG3150.018.7936524
TAGGTCG2500.018.521
AATGCGA3100.018.520
GTACTAG3100.018.51
ACGAGTC3100.018.526
CTGTGCG4550.017.4835179
ATGCGAC3500.016.91428621
ATAGTCG700.002593853715.85714230
TTAACGG957.065993E-515.57894835
ATCGCGT1101.4534049E-515.13636422
CCGTCGT2450.015.102049
GTCGTCA3050.014.55737724
CGCGTAT1152.2121783E-514.47826124
TAGTACT4500.014.3888894
CATTGCG3100.014.32258129
GAACCGT1551.2122109E-714.3225816
CGTCGTA2600.014.23076910
ATCGTCA3800.014.11842210
Produced by FastQC (version 0.11.5)