FastQCFastQC Report
Thu 28 Apr 2016
ERR1042399.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042399.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3692750
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT255290.6913276013810845No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC238220.6451018888362332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174330.472087197887753No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG156550.42393879899803666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT149230.40411617358337276No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA143560.38876176291381764No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC105430.28550538216776117No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG89610.2426646807934466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76290.2065940017602058No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG57840.1566312368830817No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT53210.14409315550741317No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC45820.12408096946719925No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA42800.11590278247918219No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG41280.11178660889580937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG450.003827387220.55555713
TATACCG951.678618E-719.4736845
TTAACGG1254.129106E-1019.2435
TAACGGC1557.223207E-915.51612936
CGAGCCG11550.013.77489215
TATACTG6400.013.58593755
TACCGTC6850.013.503657
CGGACAT8500.013.49411821
TTAGACT3200.013.2968754
ATAACGC7550.013.2317893
TATAACG2254.0745363E-1013.1555562
TATTCCG1857.397466E-813.05
ACCGTCG6850.012.9635048
TTTCGCG1000.001833815512.95000128
CTACGCT1000.001833815512.9500014
ACACCGT3000.012.9499996
CGAACGT3050.012.7377054
AAGACGG8300.012.704825
ATACCGC11950.012.69456127
CGTCGTA7050.012.59574510
Produced by FastQC (version 0.11.5)