FastQCFastQC Report
Thu 28 Apr 2016
ERR1042398.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042398.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570371
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85121.492361988951051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.7451290475848176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA38780.679908340360923No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31270.548239654540641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.5180838436736791No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24040.42148005421032975No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23960.4200774583560525No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17390.3048892738235289No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG17120.30015551281534303No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC13300.23318156077360175No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10890.19092836066349797No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT9420.16515566184115252No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.1463959422901936No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG7840.13745439371917575No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.13096738789314324No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.12658427584852666No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.12658427584852666No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG7180.125882977921388No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC7070.12395440862175672No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC6470.11343493971467694No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC6110.10712325837042908No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT6080.10659728492507507No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5900.10344144425295115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACT555.1387295E-420.1818184
CTCGAGT656.89434E-519.92307728
TAGACTG609.229038E-418.55
GTGTAGA1103.8415237E-818.51
TATACTG902.1483502E-618.55
CCCTATG500.00703064418.4999987
TATAGAC500.00703064418.4999983
GCCGTCA500.00703064418.49999813
AGGTCGC500.00703064418.49999815
ACGGACC1351.1477823E-917.8148168
GTTAATA852.7192233E-517.4117642
TAGATAA752.0649667E-417.2666665
GTCGCTG650.001578614817.07692322
TCGAGTT650.001578614817.07692329
GCGCAAG1655.4569682E-1116.8181821
GACGGAC1553.9835868E-1016.7096797
CGCAAGA1352.212255E-816.4444452
GCTCGAG904.439641E-516.44444527
TGCGCCA803.3788284E-416.187510
TTAGAAC700.002590346215.8571433
Produced by FastQC (version 0.11.5)