FastQCFastQC Report
Thu 28 Apr 2016
ERR1042397.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042397.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873401
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC531982.839648318752899No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG382362.04099389292522No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA331301.768441460210601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT327431.7477838433949806No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT231041.2332650617780176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230541.2305961190369814No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG222121.1856511232779314No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC195841.0453714928090676No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG150210.8018037782621019No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT133710.7137286678079066No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC118250.6312049582550666No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG107670.5747301298547401No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104070.5555137421192793No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA98310.524767521742542No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90970.4855874423041303No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC76490.4082948605237213No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT72250.3856622260797341No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG68680.36660597490873553No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG58870.31424131832960484No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT58570.31263995268498307No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC57910.3091169482668153No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT56820.30329865309135634No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT56610.3021776971401211No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA56600.3021243182853004No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG55740.2975337367707181No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA55090.29406411120737097No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC54170.2891532565638643No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.27933154727685106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47400.2530157718502339No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47330.2526421198664888No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC45310.24185959119270248No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT42860.228781771761625No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT41440.22120197437708208No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT41420.22109521666744064No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG40060.21383569241182213No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38910.20769712410743882No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC38440.20518831793086478No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT37320.19920988619094365No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG36750.19616729146616235No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC34480.18405029142185791No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT34200.1825556834868776No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC34060.18180837951938747No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT31550.16841028695938565No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29500.15746762172113712No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29260.15618652920543974No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26950.1438560137418524No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25740.13739717230854473No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT25590.13659648948623387No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCT25510.13616945864766808No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC25320.1351552604060743No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA25050.13371403132591475No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT24010.1281626304245594No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT22800.12170378899125174No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC22400.11956863479842277No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGT22250.1187679519761119No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA18990.10136644530455573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCA701.9242725E-723.78571524
TCTAACG551.9034891E-523.5454542
CGCAATA551.9034891E-523.54545436
CTGTGCG1200.023.1250029
TGCGCGA652.6843463E-622.7692312
TACGGTG502.7029827E-422.228
CGCGATA603.727899E-521.58333414
CTAACGC603.727899E-521.5833343
CTAGCGG555.144758E-420.18181829
CATTGCG902.153689E-618.529
CAATTCG500.00703649618.515
TAACGCC902.153689E-618.54
CGGGCGT701.2197173E-418.56
GGTCGTC1054.801259E-717.61904723
CGACGAG852.7245982E-517.41176424
GGCGTCG752.0681423E-417.2666668
GTCTAAC752.0681423E-417.2666661
ATCGTGG752.0681423E-417.26666634
TCGTGGT752.0681423E-417.26666635
CTTGCGG752.0681423E-417.26666634
Produced by FastQC (version 0.11.5)