FastQCFastQC Report
Thu 28 Apr 2016
ERR1042396.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042396.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685601
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC414842.4610806471994264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT340162.0180339238052185No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG292671.7362946509879857No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA273151.6204902583707534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT218731.2976380531335707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214611.2731957325606713No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG177781.0546979979247757No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC162250.9625646876099385No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG138000.8186990871505179No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT117980.6999283934928847No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC96300.5713095803811222No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG94650.5615207869478008No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA89140.5288321494825882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84590.5018388100149442No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT77350.458886770949946No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC66600.3951112985813369No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT65510.3886447623132639No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG57160.3391075349385768No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG54740.3247506379030387No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT52780.3131227378246691No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA51980.3083766561600284No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC50470.2994184270180191No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA47490.28173927281723254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC47320.2807307304634964No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG44760.2655432691366462No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC42730.2535000869126205No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG39180.23243934952577747No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC38540.2286424841940649No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC38480.22828652806921684No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT38430.2279898979651768No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG35630.2113786121389344No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT35520.21072602591004636No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.20509005393328553No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT34510.20473409780843746No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT34110.20236105697611714No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT34010.20176779676803705No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33420.19826756154036454No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA32760.19435204416703597No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT31690.18800415994057906No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC29130.17281669861372886No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT29050.1723420904472648No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG28670.17008770165656048No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT27330.16213801486828733No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26530.15739193320364664No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26050.15454428420486224No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT24780.14700987956224515No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC24610.14600133720850902No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG24200.14356897035538066No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23390.13876356266993198No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21560.1279069008620664No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20730.12298284113500171No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC18900.11212617932713613No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG18570.11016842064047186No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC17710.10506638285098312No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA17420.10334592824755087No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGT17330.1028119940602788No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA17200.1020407557897747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTCG502.7028276E-422.19999920
CACGCAG2150.020.6511655
CGATAAC450.003826537620.55555510
ACGACGG555.1444623E-420.1818186
CTAACGC656.9045745E-519.9230773
GAGCGTA656.9045745E-519.9230776
TCTAACG656.9045745E-519.9230772
TTGCGTG500.00703621218.4999987
GTACTCC8250.017.71515336
TATCACG2550.017.4117642
GTCTAAC752.0679872E-417.2666661
CTAGTAC1401.87174E-917.1785723
TCGGACC650.001580347917.07692313
ATCGGGC650.001580347917.07692322
GCGTCTC650.001580347917.07692312
TCACGCA2750.016.8181824
TAGGTCG1107.8129233E-716.81818221
CGCCAAT1107.8129233E-716.81818214
GGGCTGT8850.016.72316437
TGTACCT9550.016.4659735
Produced by FastQC (version 0.11.5)