FastQCFastQC Report
Thu 28 Apr 2016
ERR1042394.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042394.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707507
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT172671.0112403638755214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122050.7147847710141159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112840.6608464855488148No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC102810.6021058771647788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG64050.3751082718840977No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA63520.3720043314610131No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC48600.284625480305498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.2828099679825617No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG35910.21030660489239575No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG34070.19953066078206416No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27890.16333754415062426No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA23030.13487499612007448No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG22580.13223957500613467No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC21530.1260902590736085No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA21080.12345483795966868No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.10506545507573321No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17840.10447980593930216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG3400.015.2352941
AGTACCG855.3660455E-415.2352945
GTGCTAC1752.240995E-914.81
TAGATCT1752.240995E-914.84
GACGGAC3400.014.6911767
CGCGGTT5100.013.78431510
TTTCGGA5150.013.65048530
GTCTTAC1907.1304385E-913.6315791
TAGTACC1102.457454E-413.4545474
ACGGACC3850.013.4545458
CGACGGT3250.013.0923077
TACGACG3250.013.0923075
ACACCGT850.00940717513.0588246
GTCTAGC850.00940717513.0588241
CGTTATT2703.6379788E-1213.0185182
TCTTTCG3000.012.9510
CGAACGA2001.4635589E-812.94999916
GTTTAGG2001.4635589E-812.9499991
CAGGACT6150.012.9349594
TACCGTC3300.012.8939397
Produced by FastQC (version 0.11.5)