FastQCFastQC Report
Thu 28 Apr 2016
ERR1042393.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042393.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8514067
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC509140.5979985828159445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT460210.540528985736194No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG340160.3995270415419564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT326500.38348300524297024No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA315500.37056321027306927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT290230.3408829176467604No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC207660.24390223849542178No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG193370.22711825030270494No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG151900.17841062326617818No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139240.1635411137826376No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT133090.1563177738676475No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC108240.12713078250382573No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA105870.12434715395121979No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG103820.12193937397955643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC16500.015.3606067
ACGGACC17500.014.6942868
AAGACGG18750.014.0106675
TCTAGCG14450.013.44290728
CGCAAGA19550.013.34271052
TATACTG14450.013.3148795
CGGACCA19150.013.2349869
CGACGGT14050.013.0355867
TTAGACT8800.012.8238644
TATATCG3900.012.8076935
TAATACT19100.012.6884814
ACGACGG14500.012.6310346
GTATTAG23050.012.6008681
CGAACGA7800.012.57051316
CTAGCGG15500.012.53225929
AGACGGA20750.012.4819286
TACGACG15150.012.4554465
CGAGCCG22900.012.11790515
CGCAATA16800.012.11309636
GCGCAAG22450.012.031181
Produced by FastQC (version 0.11.5)