FastQCFastQC Report
Thu 28 Apr 2016
ERR1042392.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042392.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913976
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT337221.7618820716665202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228891.1958875137410292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT212321.1093138053977687No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC147010.7680869561582799No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG97160.507634369500976No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA92620.48391411386558664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91550.4783236571409464No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC60340.3152599614624217No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG57250.29911555839780646No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG54470.2845908203655636No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA51050.26672225775035846No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT42620.22267781832165084No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37100.19383733129360034No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG37020.19341935322073003No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA35410.1850075445042153No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.18145473088481778No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC32390.16922887225336158No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG28210.14738951794588856No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT27890.14571760565440736No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27560.1439934461038174No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.139918159893332No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC24840.12978219162622728No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24620.12863275192583398No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.11781756929031503No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20530.10726362295034003No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC20000.1044945182175743No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG19810.10350182029450734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC450.00382672420.5555573
TATAGCG500.00703655218.4999982
TGCGACG1107.8142693E-716.81818222
ACGTGCG803.3840365E-416.18759
TATATCG855.3664664E-415.2352945
GTATTAG3100.014.9193541
TTACTCG750.004105733214.89
AATCGTC750.004105733214.828
ACCGACC1152.2112525E-514.4782618
ACGGACC1152.2112525E-514.4782618
TCTAGAC1702.4392648E-814.1470593
GTATACG1458.918105E-714.0344831
GTACTAG1854.9039954E-914.01
GTTCTAA2551.8189894E-1213.7843131
CCTATAT3650.013.6849321
GTTCTAC1652.597426E-713.4545451
GTATATG3050.013.3442621
AGTACTC6600.013.1742425
TATAACG2254.0745363E-1013.1555552
CGTCTAA850.00940771913.0588241
Produced by FastQC (version 0.11.5)