FastQCFastQC Report
Thu 28 Apr 2016
ERR1042391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871908
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT456092.4364979475487045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313221.6732659938415777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306811.6390228579609682No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC168160.898334747220483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132460.7076202462941555No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG115930.619314624436671No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA103390.5523241526827173No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC73650.3934488233396085No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG66000.35258143028396693No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA64240.3431792588097278No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG60770.3246420230054041No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49020.2618718441290918No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.2563160155306778No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40020.21379255818127813No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39960.2134720296082927No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA38940.20802304386754047No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC38750.20700803671975335No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.20097141526186113No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA35610.19023370806684944No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.18264786517286105No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT33780.18045758659079397No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31860.17020067225526042No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29260.15631110075922536No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA28590.1527318650275548No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC28000.14958000072653144No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.13830807924321067No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG24030.12837169348066252No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA23410.12505956489314646No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA22810.12185427916329222No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG22440.11987768629654877No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20600.11004814339166241No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT20430.10913997910153704No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT19980.10673601480414635No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA19640.10491968622389561No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT19580.1045991576509102No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.10262256478416674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC18810.10048570763093057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA806.9649286E-720.81259
TAGGTCG1001.2911187E-820.3521
GTGCGCG759.270874E-619.73333411
CGACGGT1205.178663E-918.57
TACGACG1205.178663E-918.55
CTAGCGG801.617555E-518.529
ACGACGG1258.587449E-917.7600026
CTAGTAC2100.017.6190473
CGTCGAG953.6088386E-617.52631610
TGTGCGC2300.016.89130410
TGCGACG803.3839856E-416.187522
TAGTACT2550.015.9607844
TGGTCCG1302.591296E-715.6538465
TCGGAAC1802.0190782E-1015.41666632
GTTTTCG1802.0190782E-1015.41666628
GAACAGT22150.015.2844251
GTATAGG2300.015.2826091
CTGTGCG2300.015.2826099
GGCGTCG855.3663895E-415.2352938
CAACCCG855.3663895E-415.23529325
Produced by FastQC (version 0.11.5)