FastQCFastQC Report
Thu 28 Apr 2016
ERR1042390.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042390.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371487
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243861.028299965380371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172890.7290362544681881No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161520.68109165262133No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC104460.4404831230363059No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71790.30272145704361864No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG66570.2807099511825281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA66010.27834856358057203No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC44620.1881519907129999No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG39170.16517062922967743No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG37080.15635759335809135No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33070.13944837142265593No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT28920.12194880258673145No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.11874406226979108No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27920.1177320390118099No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG25920.10929851186196678No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA25590.10790697988224265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1155.456968E-1222.5217435
TAACGGC1303.274181E-1119.92307736
GTACTAG1054.802587E-717.6190471
CGAACTA1059.3514045E-615.85714229
TTAGACT3050.015.1639354
ACCGATC750.004106116514.88
GTCTTAC2500.014.7999991
TTCGCCG1252.9617731E-614.79999916
TATATCG1651.6474587E-814.5757575
ATACCGA908.278587E-414.3888896
GTATAGG6500.014.2307691
CGACCGT1051.6568034E-414.09523821
TTACGGT800.006301225613.8754
GCGGTAC2151.9826984E-1013.76744237
CGAACGA1753.570858E-813.74285716
ACGAACG1753.570858E-813.74285715
GTAAGAC2700.013.7037033
TCTATAC3250.013.6615383
TAGGGGT4200.013.6547614
GTACTAA1501.3070585E-613.5666671
Produced by FastQC (version 0.11.5)