FastQCFastQC Report
Thu 28 Apr 2016
ERR1042389.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042389.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2720777
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT531991.9552870374896583No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC494571.8177527963519247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT342271.2579862296689512No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA332221.221048252025065No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG331391.2179976528763659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327271.202854919752703No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG201190.7394578828033316No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC192760.7084740866304001No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG174450.6411771343259665No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT148200.5446973419725322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148040.5441092746667587No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG114900.4223058339584611No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA113810.4182996254378804No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC110780.4071631008347983No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT91950.3379549297866014No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC86580.31821792083658457No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT84240.3096174364896498No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA71370.26231477258150887No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65920.24228372997860537No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC65050.239086114003463No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG64540.23721164946631051No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT64380.23662358216053722No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG60610.2227672462682535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC60240.22140734062365275No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58530.21512237129320044No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA54990.20211138215296587No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG53230.19564264178945942No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50240.1846531340128206No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46590.17123784859986688No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT46160.16965741771560108No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC45970.16895908778999527No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT44870.16491612506280376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT44560.16377674465786796No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC42240.15524976872415489No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG41550.1527137284680075No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCT41260.15164785647629336No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA40590.1491853246333676No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC39720.14598770865822522No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCT39220.1441499983276836No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38800.14260632165002865No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT38000.1396659851211621No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT36940.1357700392204139No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34250.12588315764210004No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC33750.12404544731155842No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG33480.12305308373306595No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG32940.12106835657608103No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA32900.1209213397496377No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA31300.11504066669190455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT29480.1083514010887331No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT29200.1073222833036298No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT28010.10294853271694078No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.10276476168388662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT656.906564E-519.92307715
GACGGAC2600.019.9230777
CGAACGA1254.129106E-1019.2416
ACGGACC2800.017.8392858
TATACTG3250.016.5076925
TGCGACG1251.6600461E-716.27999922
AAAAGTA12700.015.5866152
CGCTTAT957.065347E-515.57894824
AAGACGG3600.015.4166665
ACGAACG1601.0983058E-815.0312515
CGGACCA3450.015.0144939
AGTACTC14100.014.9574475
TACTAGG2600.014.9423072
GGCGTGT750.00410632414.88
TAAACGT750.00410632414.84
CTAGTCC2251.8189894E-1214.83
CACGCAG3650.014.6986315
TACCTGT18600.014.62096837
GTACTAG1904.5656634E-1014.6052641
GAGTATA1651.6478225E-814.5757591
Produced by FastQC (version 0.11.5)