FastQCFastQC Report
Thu 28 Apr 2016
ERR1042388.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042388.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7125344
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC876771.230494976803927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT746181.0472196149406963No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG567880.7969860823561641No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA548010.7690997094315727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT501410.7036993582344936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT442950.6216541966254542No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG329020.4617601620356856No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC320750.4501537048597233No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG278340.39063377150633005No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT237060.3326997264974154No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228840.32116344137209374No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC189800.2663731042318799No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA185890.26088564987178164No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG175460.24624776010814353No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT146720.2059128653999021No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC139440.19569581482662451No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139040.19513443842149936No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT131520.1845805620051467No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG129080.18115616593388334No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109710.15397151351569832No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC104050.14602803738317757No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA102420.1437404285322926No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG101010.14176157670422648No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT99870.14016165394961985No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA95560.13411282318439643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC94640.1328216574526086No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGT94260.13228834986773974No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG92440.1297340872244203No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT90310.12674475786712894No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC83600.11732766867115468No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC81160.11390327259989132No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG79370.1113911131869563No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC73450.10308274239110421No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG72760.10211436809226333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTC24950.014.3106215
GTATACG3450.013.9420291
AAGACGG14250.013.8912285
ACGGACC12900.013.6240318
GGACCGT2601.8189894E-1213.5192316
AAAGTAC25850.013.4545463
AAGTACT28250.013.424784
TCTAGCG9400.013.38297928
GACGGAC12950.013.2857147
GTGTAAG9150.013.1420761
CTAGCGG9750.012.90256529
TATACTG10650.012.854465
CGAGCCG14850.012.8316515
CAAGACG14800.012.754
TACGACG8100.012.3333335
TACCTGT38500.012.20519537
GTACTAG4700.012.2021281
GTAAACG7750.012.17419427
TGTACCT36050.012.05963935
CGCAATA10600.012.04245236
Produced by FastQC (version 0.11.5)