FastQCFastQC Report
Thu 28 Apr 2016
ERR1042386.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042386.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10065935
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299030.2970712606429507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT236780.23522901747328986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT209230.2078594785283235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.019.5969561
TCTAGCG13550.015.83763828
TCTATAC13450.015.6802973
CTAGCGG14100.015.35106429
ACGGACC18550.013.9622648
TATACTG15450.013.8899675
GTATCAA180200.013.520812
TACGACG17750.013.3408445
GACGGAC19850.013.2342577
AAGACGG20750.013.1951815
TCGTTTA16050.013.14018630
ATAACGC21300.013.0281693
AGACGGA21450.012.8508166
GTAAACG15150.012.82178227
CGAACGA14150.012.8127216
TAACGCC17650.012.7875364
CGTTATT16800.012.7738092
CGGTCCA17550.012.75498610
TCGCTAA4950.012.70707114
TTAGACT10800.012.6759254
Produced by FastQC (version 0.11.5)