FastQCFastQC Report
Thu 28 Apr 2016
ERR1042385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7904149
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC116800.14777049369894216No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA111250.1407488649315695No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC89950.11380099236489595No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC84210.10653898351359521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC18300.017.1857918
GACGGAC18450.016.845537
GGTATCA52150.016.7794841
AAGACGG20550.016.7445265
TCTAGCG13200.016.53787828
CGCAAGA19950.016.4135342
CGAACGA14900.015.768455516
TAACGAA15100.015.6821213
TATACTG9450.015.6613745
CGGACCA20850.015.6163079
ATAACGA15550.015.5852112
TTAGACT6700.015.4626874
AGACGGA21750.015.3103466
CTAGCGG14500.015.05517229
TCGTTTA14150.014.77385130
GCGCAAG23450.014.5948831
ACGAACG16450.014.39513615
GATAACG17450.013.99426911
CGCAATA16050.013.94704136
ATTAGAC6400.013.8753
Produced by FastQC (version 0.11.5)