FastQCFastQC Report
Thu 28 Apr 2016
ERR1042384.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042384.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7971887
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT779130.9773470195952351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT652840.8189278147068567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT523680.6569084584365031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267960.33613120707807326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA317500.016.029451
TCTAGCG8600.015.05813928
ACGGACC16400.014.8902448
CTAGCGG8850.014.84180729
AAGACGG16800.014.7559525
GACGGAC16500.014.5757587
TATACTG10800.014.3888895
GTACGTA5250.013.74285813
TCGTTTA13200.013.45454530
CGGACCA18200.013.3159339
CTACGCT1951.0288204E-813.2820524
CGCAAGA18700.013.1577552
AGACGGA18650.012.9946386
TAACGCC15400.012.9740264
GTAAACG12000.012.79583427
TAAACGC12300.012.78455328
CGCATCG13950.012.73118213
TACCGTC13000.012.6653847
TAATACT20500.012.5439024
GTCGGGA9450.012.52912
Produced by FastQC (version 0.11.5)