FastQCFastQC Report
Thu 28 Apr 2016
ERR1042383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11578715
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT739650.6388014559474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT606190.5235382337331905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT528570.45650143388104814No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265530.22932596579154077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA283950.018.0146141
ACGGACC13300.015.8571438
GACGGAC14300.014.3601397
CTAGCGG8950.014.2625729
GTATTAG43650.014.0286371
TCTATAC22950.013.8649243
TCTAGCG9250.013.628
AAGACGG16850.013.394665
CGGACCA15800.013.3481019
TTAACGG7350.013.34013735
AATCGTC12900.013.19379928
CGGGTAA10850.013.12903224
TTTAGCG9350.012.86096226
TAATACG13100.012.8511464
CGCAAGA16000.012.8343742
CTAGTAC7700.012.73376753
GTATCAA403700.012.6663362
CGCCGTA8200.012.63414725
TTAGACT14450.012.5467134
ATGTACG14650.012.50170611
Produced by FastQC (version 0.11.5)