FastQCFastQC Report
Thu 28 Apr 2016
ERR1042382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4881767
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT367070.7519203599844073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT326800.6694297372242469No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176790.36214346157856364No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122430.25079033882608487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC11050.017.9140267
ACGGACC11200.017.5089288
CGTTCGG1502.5283953E-1017.26666624
CGCAAGA12100.016.6652892
AAGACGG12500.016.5765
CGGACCA12400.015.9637099
GGTATCA146350.015.6368291
TATACTG7850.015.3184715
GCGCAAG13600.015.2352941
CGTCGTA7200.014.90277810
AGACGGA14050.014.8790046
TCGTTTA8600.014.84302330
ATGGTCG8200.014.66463436
TACCGTC7700.014.6558447
TGGTCGG8900.014.1348337
TCGGTAA2009.858923E-1013.87500127
TACGAAC3500.013.74285728
CCGTCGT7850.013.668799
CGGTAAG1907.1431714E-913.63157928
TCGAACG2457.2759576E-1213.5918363
Produced by FastQC (version 0.11.5)