FastQCFastQC Report
Thu 28 Apr 2016
ERR1042381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932548
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT329710.8384131611362404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT261390.6646835588529371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114530.2912361146005084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98490.2504483098489834No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT65710.16709268392909635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA5900.018.81355916
ACGGACC13950.018.5663098
GACGGAC14050.018.4341627
GGTATCA117050.017.859891
AGACGGA15500.017.6645156
AAGACGG14850.017.4410765
CGGACCA15200.017.1611849
ACGAACG6600.017.09848415
CAAGACG17200.016.671514
CGCAAGA16250.016.282
GCGCAAG17000.015.9970591
TATACTG6550.015.8167945
GTATCAA140050.014.9136022
GTATACT4100.014.8902444
GCAAGAC21400.014.782713
GCGGGTA4950.014.57575823
TAACGAA8050.014.24844713
AACGAAC7800.014.2307714
CGAACGT3000.014.1833324
TAGACCG800.006302199313.8755
Produced by FastQC (version 0.11.5)