FastQCFastQC Report
Thu 28 Apr 2016
ERR1042380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11222767
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT576250.5134651730718458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT422240.3762352011763231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT412710.3677435341925926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216940.1933034874554555No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA113020.1007060023610933No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC112970.10066145006841895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231450.018.4800171
AAGACGG24750.017.2666665
TCTAGCG14950.016.08695828
GACGGAC24450.015.889577
ACGGACC25350.015.6173568
CTAGCGG15550.015.34726629
TTAACGG5500.015.13636435
CGCAAGA27200.014.7591912
AGACGGA28150.014.392546
TATACTG18350.014.3160765
CGGACCA28400.014.2658459
TCGTTTA16650.013.88888930
TAACGGC6000.013.87536
GCGCAAG32300.013.2879261
CGAACGA18000.013.15555616
GTATTAG34800.013.0244251
TCTATCG7150.012.67832231
GTATCAA336200.012.661662
CAAGACG37000.012.64
TCTATAC14300.012.419583
Produced by FastQC (version 0.11.5)