FastQCFastQC Report
Thu 28 Apr 2016
ERR1042379.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042379.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10070662
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT625780.6213891400585185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT498100.4946050219935889No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT459090.455868740307241No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257990.25617978242145356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5300.018.84905635
TAACGGC5800.017.22413836
AAGACGG20900.017.0837335
TCTAGCG13050.017.01149628
GACGGAC19800.016.8181827
ACGGACC20150.016.8014898
GGTATCA287850.016.6201151
CGCAAGA21800.016.038992
CTAGCGG14400.015.93055629
CGGACCA23100.015.1363649
CGAACGA14350.014.95470316
AGACGGA23550.014.3757966
GCGCAAG25100.014.2988041
CAAGACG31400.013.668794
TATACTG15850.013.4227125
CGGTCCA17450.013.35816710
CGCAATA17500.013.21428536
GTATTAG35750.013.1440551
ATAACGA16650.013.012
ATACCGC22800.012.98245627
Produced by FastQC (version 0.11.5)