FastQCFastQC Report
Thu 28 Apr 2016
ERR1042378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998421
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT353990.5058140972084989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285250.40759194109642727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265050.3787282874236917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144390.20631796801021257No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA80090.11444010013115817No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC78460.11211100332489286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG9950.018.03517528
CTAGCGG10100.017.95049529
GGTATCA148850.017.922071
AAGACGG15850.017.7413275
CGAACGA9850.017.27918616
GACGGAC16450.015.85714157
ACGGACC17050.015.6246348
TTAACGG3400.015.23529435
TAACGGC3300.015.13636436
GCGTAGA2006.184564E-1114.81
ATAACGA11800.014.73728812
CGGTCCA12450.014.71084310
CGCAATA12450.014.56224936
AGACGGA19100.014.1413616
ACGAACG12100.014.06611615
CGGACCA19600.014.0637759
CGCAAGA18950.014.0580472
TAGCGGC13650.013.95970730
GCGCAAG20150.013.8635241
CAAGACG21750.013.779314
Produced by FastQC (version 0.11.5)