FastQCFastQC Report
Thu 28 Apr 2016
ERR1042377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7521040
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT278340.3700817971982598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT219620.29200748832608253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211500.2812111091019327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106040.14099113952325742No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA85070.11310935721655516No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC82680.10993160520353569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100900.023.1387521
CTAGCGG10600.017.27830129
TCTAGCG10500.017.26666628
TATACTG12900.016.779075
CGAACGA11300.016.69911616
AAGACGG16750.016.0149255
TCGTTTA12350.015.27935330
TTAACGG3900.015.17948735
AATCGTC4650.015.11827928
ATAACGA12600.014.9761912
GTATCAA156000.014.9660252
TCTATAC12100.014.8305783
ATAACGC15950.014.6144213
GACGGAC17750.014.5915497
ACGAACG12950.014.57142815
CGAGCCG20350.014.36363615
ACGGACC17700.014.3192098
TAACGCC13400.014.2201494
TAACGAA13400.014.22014913
CCTATAC19150.014.104443
Produced by FastQC (version 0.11.5)