FastQCFastQC Report
Thu 28 Apr 2016
ERR1042376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196746
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT174481.4579534838637438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT126661.0583699465049392No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74950.6262816002727396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50290.4202228375946107No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT26690.22302142643468204No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.14372306237079546No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.14372306237079546No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16830.1406313453314237No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG12430.10386498053889463No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.101274623019421No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12000.10027190397962475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG250.005496433529.59999826
CGCATAT400.001931232923.1250021
TTCCGTA400.001931232923.12500234
ACGGACC5800.020.7327588
AAGACGG6200.020.2903215
CGGACCA6200.020.2903219
GCTACCC555.1432644E-420.1818183
GACGGAC6000.020.0416667
TAATCGG656.902433E-519.92307716
CGCAAGA6250.019.8322
GGTATCA56200.019.6521361
GCGCAAG6350.019.5196861
CAAGACG6750.019.1851844
CCGTCGT1800.018.59
TAACACG1103.852074E-818.54
GGTATAG801.6167605E-518.51
CGTCGTA1800.018.510
TTCGCGG1103.852074E-818.532
TCTATGC609.237118E-418.58
AGACGGA6650.018.3609036
Produced by FastQC (version 0.11.5)