FastQCFastQC Report
Thu 28 Apr 2016
ERR1042375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7328695
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT811481.1072639808315123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT674700.9206277515983404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT606010.8269002871589006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331700.45260445413542244No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA164060.2238597731246832No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93790.12797639961821305No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.12680293012603197No Hit
GAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAA77460.10569412426086773No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77310.1054894493494408No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAA75820.10345634522926661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC13600.018.0919118
GACGGAC13850.017.2310477
AAGACGG15900.016.8710695
AGACGGA16400.015.3414646
CGGACCA16050.015.3302189
TATACTG10000.015.1700015
CGCAAGA16650.015.111112
GGTATCA382300.014.3044731
CTAGCGG7650.014.26797429
ATCGTAC2750.014.12727425
GCGCAAG18600.014.1236551
GTATTAG19850.013.886651
TCGTTTA10950.013.85388130
TCTATAC9850.013.7106593
CGAACGA9300.013.52688116
TACGACG12900.013.480625
TACCGTC11500.013.3521737
CGACGGT13200.013.3143947
CGAGCCG15300.013.30065315
TCTAGCG7950.013.26415128
Produced by FastQC (version 0.11.5)