FastQCFastQC Report
Thu 28 Apr 2016
ERR1042374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9640186
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT486780.504948763436722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT378910.3930525821804683No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT351810.3649410913855811No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174460.18097161195852446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG13050.018.9961728
CTAGCGG13550.018.29520229
GGTATCA201050.017.6948531
GACGGAC21500.017.4674427
ACGGACC21650.017.431878
AAGACGG23200.016.9849135
CCGGTCC17050.015.7331389
CGGACCA24100.015.5062259
AGACGGA24450.015.3599196
CGCAAGA24700.015.0546552
TACGACG16350.015.0489315
CGGTCCA17300.014.75722510
ACACGCT31850.014.6373639
TCGTTTA15750.014.5650830
CGCAATA18200.014.43406636
TATACTG17050.014.4310865
TTAACGG6550.014.4045835
GCGCAAG28100.014.0889681
TATATCG8350.013.9580845
TAACGGC6800.013.87536
Produced by FastQC (version 0.11.5)