FastQCFastQC Report
Thu 28 Apr 2016
ERR1042373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11715585
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT997780.8516689520839121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT881580.7524848311031843No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT781410.6669833388601594No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478010.4080120625645241No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160170.13671532407472609No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147340.12576409970138067No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT117950.10067785774248575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG15200.015.94407928
CTAGCGG15850.015.406939529
TCGTTTA16600.015.26807230
ACGGACC21750.015.0551728
TAACGCC19200.014.7421874
GTAAACG19250.014.41558427
CGAACGA14950.014.35451416
GACGGAC22100.014.314487
AAGACGG24250.014.1896915
TAAACGC20350.014.09090928
CTAGTAC6550.013.8396953
ATAACGA15800.013.69936712
AACGCCG19900.013.6658295
GTATTAG34950.013.6037191
GGTATCA538750.013.5466361
ACGAACG16200.013.47530815
TATACTG21350.013.4309135
CCGGTCG20000.013.41249920
CGCATCG19900.013.38693413
CGTTTAT19800.013.17424331
Produced by FastQC (version 0.11.5)