FastQCFastQC Report
Thu 28 Apr 2016
ERR1042372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308767
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT429370.5167674096529605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT320740.3860259891750485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289230.34810219133597076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155590.18726003509305292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG9700.016.78350429
GGTATCA185300.016.3634641
TCTAGCG9950.016.1758828
ACGGACC16600.015.8253018
GACGGAC17000.015.4529427
AAGACGG19150.014.780685
TTAACGG3400.014.14705935
GTATTAG22950.013.5424841
TAATACT20800.013.519234
CGGACCA20050.013.2867849
CGCAAGA19900.013.2010062
AGACGGA20500.013.0853666
CGCAATA12900.012.90697636
GCGAAAG19400.012.77835118
CGTTCGG1901.04852006E-712.65789524
GTATAGG15350.012.5342021
TACCGTC12700.012.5275597
TAACGGC3850.012.49350636
TTCGGGC11700.012.33333435
GTAAACG10800.012.33333427
Produced by FastQC (version 0.11.5)