FastQCFastQC Report
Thu 28 Apr 2016
ERR1042371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11356624
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT526210.4633507281741475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT436700.3845332908794022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT364180.32067628548765903No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189140.16654597352170855No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA116660.10272418986487535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC24850.017.1971848
TCTAGCG15800.016.74367128
GACGGAC25100.016.7310757
AAGACGG26600.016.2048875
GGTATCA230250.016.1337681
CTAGCGG17150.015.42565629
CGGACCA27950.014.9588559
AGACGGA28700.014.7613256
CGCAAGA29150.014.66037752
TAACGCC20450.014.4743274
TTAACGG5300.013.96226435
TCGTTTA19800.013.92171830
TACGACG19550.013.8158565
TATACTG18950.013.5699215
CGCAATA20200.013.55445636
GCGCAAG32950.013.362671
GTATACG5400.013.3611111
GTACTAG7350.013.3401371
CGCATCG21150.013.29550713
CGAACGA15200.013.26644816
Produced by FastQC (version 0.11.5)