FastQCFastQC Report
Thu 28 Apr 2016
ERR1042370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7003680
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT367650.5249383181413201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT302700.43220135700089096No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT267120.38139949283805086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140420.20049459712608228No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA86590.12363500331254428No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC83150.11872329975098805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA11250.018.0888916
CTAGCGG8950.016.9497229
ACGAACG12500.016.2815
TTAGCGA3200.016.187527
GACGGAC14550.016.1477667
AAGACGG16050.016.1370725
TCTAGCG9350.016.02673728
GGTATCA174850.015.7755211
TTAACGG2950.015.67796635
ACGGACC15100.015.5596048
ATAACGA12750.015.5254912
TAACGAA13150.015.19391613
CGGACCA16400.014.8902449
CAAGACG19300.014.4740934
AACGAAC13900.014.37410114
GTATTAG22500.014.3066671
GATAACG14250.014.15087711
AGACGGA16750.014.1373136
CGCAAGA16900.014.1213012
CCGATAA14650.013.7645059
Produced by FastQC (version 0.11.5)