FastQCFastQC Report
Thu 28 Apr 2016
ERR1042369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9873464
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT264440.2678290010476566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197920.20045649632185825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196000.19851189005196151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113150.020.4865231
TCTAGCG11800.016.14830628
CGAACGA11150.015.76233216
CTAGCGG12650.015.35573229
ACGGACC17750.014.7999998
CGCAATA13900.014.77338136
GACGGAC18050.014.0415527
TATACTG18650.013.7882035
GTATCAA167600.013.7756562
ACGAACG12600.013.65476215
GTATTAG22450.013.4320711
TACCGTC15550.013.2057897
TAACGAA13750.012.91636413
ATACCGT18400.012.6684796
AAGACGG22650.012.5783665
CGATAAC14650.012.37542610
AACGAAC14000.012.28928614
TCTATAC13550.012.2878233
TCGTTTA12450.012.18473930
CCGATAA15100.012.1291389
Produced by FastQC (version 0.11.5)