FastQCFastQC Report
Thu 28 Apr 2016
ERR1042368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778517
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT255770.328815891255364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT192840.2479135804421331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181090.23280787327455862No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA92080.11837732050980926No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC81920.10531570478022996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85450.023.2088931
AAGACGG17900.017.1564255
CTAGCGG11350.015.97356729
ACGGACC17900.015.9162028
TCTAGCG11600.015.62931128
GTATCAA129250.015.2580282
GTAAACG11250.015.12888927
GACGGAC19050.015.0524957
TCGTTTA14300.015.00699330
CGGACCA20200.014.561889
TAACGCC15950.014.4984324
CGCAATA13150.014.209125536
CAAGACG22250.014.1348314
TAGACAG11450.014.0567695
AGACGGA21100.014.0284376
ATAACGC17400.013.9281623
TATACTG13450.013.8921935
CGCATCG15900.013.84591113
CGTTTAT15500.013.84516231
TAAACGC12350.013.78137628
Produced by FastQC (version 0.11.5)