FastQCFastQC Report
Thu 28 Apr 2016
ERR1042367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4996224
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT263140.5266777470345605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT204270.40884876258550457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179140.35855077754720366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79250.15861978966515514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95300.018.092341
ACGGACC9150.017.792358
CGGACCA10100.016.3019819
TCTATAC6800.015.7794123
GACGGAC9750.015.7487177
CGTTATT8000.015.0312512
AAGACGG11250.014.85
GCGTTAT8450.014.668641
GTATAGA8100.013.9320981
CGCATCG7950.013.72955913
TGACCCG8650.013.687860513
CTAGTAC2304.0017767E-1113.6739133
AGACGGA11650.013.6566536
CGCAAGA11800.013.6398312
TCTAGCG5800.013.39655328
CGGACAT19150.013.33159321
TCGTTTA8050.013.32919330
TATACTG8200.013.3109765
TAACGCC8100.013.2469144
CGAGCCG9650.013.2279815
Produced by FastQC (version 0.11.5)