FastQCFastQC Report
Thu 28 Apr 2016
ERR1042366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12425181
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT584480.4703995861307775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT453050.36462245499683266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT423900.3411620321667749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207010.16660521886964866No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA136730.11004266255759171No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC135830.10931832703282149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207150.020.4156421
ACGGACC25650.016.083828
GACGGAC26200.015.8167937
TCGTTTA18850.015.70291830
AAGACGG30750.014.8601625
TATACTG23700.014.5189895
TCTAGCG14250.014.41052628
CGAACGA17100.014.17251516
TACGACG18100.014.1049725
CGGACCA29750.013.9294129
CGCAAGA30850.013.8525112
GTATCAA306800.013.778522
CTAGCGG14800.013.75000129
GCGCAAG32250.013.480621
CAAGACG34350.013.4643374
ATGGTCG20500.013.35609836
AGACGGA32400.013.3040126
CGTTTAT22300.013.27354231
TCTATAC15700.013.1974533
GTATTAG30850.013.13291
Produced by FastQC (version 0.11.5)