FastQCFastQC Report
Thu 28 Apr 2016
ERR1042365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10375629
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT503410.4851850427574077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT352320.33956495553185256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT340740.3284041863871578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156990.15130648946680728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145300.023.74571
GTATCAA216550.015.9071822
TATACTG20300.015.2192125
ACGGACC15150.013.798688
GACGGAC15300.013.4215697
AAGACGG18150.013.2506895
TCTAGCG8550.012.98245628
TCTATAC15200.012.9013163
TAACGCC11150.012.77578454
GTATTAG24500.012.7612241
TAATACT24800.012.5322574
CGAACGA9750.012.52307716
ATCAACG276100.012.5030784
AACGCAG277200.012.4734847
CGCAAGA17100.012.4415212
TCAACGC280050.012.2936985
CAACGCA285100.012.1018946
TATCAAC286200.012.1006293
GTACATG324900.012.0429371
CGGACCA17850.012.0224099
Produced by FastQC (version 0.11.5)