FastQCFastQC Report
Thu 28 Apr 2016
ERR1042364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17003006
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA176380.103734598458649No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC174450.10259950505222429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105650.018.9640311
TCTAGCG32100.017.34735328
CTAGCGG34000.016.48676529
AAGACGG43050.016.4157965
ACGGACC40900.016.2836198
GACGGAC40900.016.1931537
TTAACGG7800.015.65384735
TCGTTTA29250.015.3692330
CGAACGA26750.014.59252416
TATACTG22650.014.5386315
CGGACCA46350.014.5285869
AGACGGA47250.014.44761856
CAAGACG52000.014.33754
CGCAAGA47150.014.2036062
CGCAATA39650.014.18411236
CGGTCCA43350.013.95501710
CGTTTAT32400.013.76080231
TAACGGC8750.013.74285736
ACGAACG28700.013.60104615
CGTTATT34550.013.5470322
Produced by FastQC (version 0.11.5)