FastQCFastQC Report
Thu 28 Apr 2016
ERR1042363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5119702
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205150.4007069161447287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160260.3130260315932451No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT134690.2630817184281429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.14461779220743706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC9500.015.9684218
CGTTCGG2150.015.48837424
CGAACGA7150.015.26573416
GACGGAC10350.015.0144927
TCTAGCG6150.014.73983828
GTATTAG16250.014.6861531
AAGACGG10900.014.4266055
CGCAAGA10950.014.360732
GGTATCA119200.014.1388421
CTAGCGG6500.013.94615429
CGGACCA11150.013.77139
TTTAGCG4050.013.70370426
CGAACGT2601.8189894E-1213.5192314
TCGGTAA2202.8558134E-1013.45454527
TAGGTCG2254.0745363E-1013.15555621
GTATTAA6350.013.1102351
ATAACGA8900.013.09550512
ACGAACG8500.013.05882315
AGACGGA12550.012.9721126
GCGCAAG13700.012.8284671
Produced by FastQC (version 0.11.5)